Description
SPECIFICATIONS:
EC 3.2.1.23
Product name: β-D-galactoside galactohydrolase
Appearance: white amorphous powder lyophilized
Activity: Grade II, 500 U/mg-solid or more
Contaminants:
- α-galactosidase ≤1×10-4 %, α-glucosidase ≤1×10-4 %
- β-glucosidase ≤2×10-3%, β-mannosidase ≤1×10-4 %
- α-mannosidase ≤1×10-4 % proteinase ≤10mAbs/mg-P
Stabilizer: Mg2+
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: 540,000
Isoelectric point: 4.61
Michaelis constants: 3.0×10-4M (o-Nitrophenyl-β-D-galactoside), 6.7×10-5M
(p- Nitrophenyl-β-D-galactoside), 2.3×10-4M (Phenyl-β-D-
galactoside), 2.5×10-3M (Lactose)
Inhibitors: p-Chloromercuribenzoate Iodoacetamide heavy metal ions
(Zn2+ Fe3+ Cd2+ Cu2+ Pb2+ Ag+ Hg2+), lonic detergents
(SDS, DAC, etc.)
Optimum pH: 7.0 – 7.5
Optimum temperature: 50 – 55℃
pH Stability: pH 6.5−8.5 (25℃, 20hr)
Thermal stability: Below 50 ℃ (pH 7.3, 15min)
Substrate specificity: The enzyme specifically hydrolyzes β-D-galactosyl linkage