Description
SPECIFICATIONS
EC 1.10.3.3
Product name: L-Ascorbate: oxygen oxidoreductase
Appearance: Light blue amorphous powder, lyophilized
Activity: Grade III, 200 U/mg solid or more (containing approx. 70% of stabilizers)
Contaminants: Catalase:< 0.1 % Phosphatase:< 0.02%
Stability: Stable at -20°C for at least 12 months
Stabilizers: Bovine serum albumin, borax, basic amino acids
Molecular weight: 132,000
Isoelectric point: Between 6.0 and 7.8
Michaelis constant: 2.5 x 10-4 M (Ascorbate)
Inhibitors: Cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH: 5.6
Optimum temperature: approx. 30 °C
pH Stability: pH 7.0 – 10.0 (25 °C, 20hr)
Thermal stability: Below 40 °C (pH 8.0, 30 min)
Substrate specificity: The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives.