Description
SPECIFICATIONS
EC 1.10.3.3
Product name: L-Ascorbate: oxygen oxidoreductase
Appearance: Light blue amorphous powder, lyophilized
Activity: Grade III, 200 U/mg solid or more
Contaminants: Catalase:< 0.1 % Phosphatase:< 0.02%
Stability: Stable at -20°C for at least 12 months
Stabilizers: Bovine serum albumin, borax, basic amino acids
Molecular weight: 140,000
Isoelectric point: 5.5 ± 0.2
Michaelis constant: 2.7 x 10-4 M (Ascorbate)
Inhibitors: Cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH: 5.5 – 6.5
Optimum temperature: approx. 45 °C
pH Stability: pH 6.0 – 10.0 (25 °C, 20hr)
Thermal stability: Below 45 °C (pH 7.0, 30 min)
Substrate specificity: The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives.