Description
SPECIFICATIONS
EC 3.1.1.13
Product name: Steryl-ester acylhydrolase
Appearance: Light brown amorphous powder lyophilized
Activity: Grade III, 100 U/mg-solid or more (containing
approx. 40% of stabilizers)
Contaminant: Catalase ≤ 1.0×10-2%
Stabilizers: Mg2+, Na-cholate, bovine serum albumin
Stability: Stable at – 20 ℃ for at least 12 months
Molecular weight: approx. 300,000
Isoelectric point: 5.9 ± 0.1
Michaelis constants:
- 5.4×10-5M (Linoleate), 6.6×10-5 M (Oleate)
- 3.7×10-5M (Linolenate), 1.5×10-4 M (Palmitate)
- 1.2×10-4M (Myristate), 2.3×10-5 M (Stearate)
Inhibitors: Hg2+, Ag+, ionic detergents
Optimum pH: 7.0 – 9.0
Optimum temperature: 40 ℃
pH Stability: 5.0 – 9.0 (25 °C, 24hr)
Thermal stability: below 55 ℃ (pH 7.5, 10min)