Description
SPECIFICATIONS:
EC 1.1.1.49
Product name: D-Glucose-6-phosphate: NADP+ 1-oxydoreductase
Appearance: white amorphous powder lyophilized
Activity: Grade III, 200 U/mg-solid or more
Contaminants:
- Creatine phosphokinase ≤ 1×10-3%
- Phosphoglucomutase ≤ 1×10-3%
- 6-Phosphogluconate dehydrogenase ≤ 5×10-3%
- Phosphoglucose isomerase ≤ 1×10-2%
- Glutathione reductase ≤ 1×10-3%
- Hexokinase ≤ 1×10-2%, Myokinase ≤ 1×10-2%
- NADH oxidase ≤ 1×10-2%, NADPH oxidase ≤ 1×10-2%
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: 140,000 (Gel filtration)
Michaelis constants: NAD+ linked: 2.4×10-4M (NAD+), 4.7×10-4M (G6P)
NADP+ linked: 7.4×10-6M (NADP+), 3.2×10-4M (G6P)
Inhibitors: Metal ions, Iodoacetamimide, SDS etc.
Optimum pH: 7.8
Optimum temperature: 50 – 55℃
pH Stability: pH 5−11 (25℃, 22hr)
Thermal stability: Below 50 ℃ (pH 7.8, 30min)