Description
SPECIFICATIONS:
EC 1.4.1.9
Product name: L-Leucine: NAD+ oxidoreductase (deaminating)
Appearance: White amorphous powder, lyophilized
Activity: Grade II, 20 U/mg-solid or more (contains approx. 70% of
stabilizers)
Contaminants: Leucylpeptide decomposing enzymes:
(Leu-Val): ≤ 1.0×10-2%, (Leu-Gly-Gly): ≤ 1.0×10-2%
NADH oxidase: ≤ 1.0×10-2%
Stabilizers: 2-Mercaptoethanol, L-cysteine, dithiothreitol,
ethylenediaminetetraacetate
Stability: Stable at -20 °C for at least 12 months
Molecular weight: 245,000
Michaelis constants: 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD＋), 3.5×10-5M
(NADH), 3.1×10-4M［α-Ketoisocaproate (α-KⅠC),
2.0×10-1M (NH3)
Structure: 6 subunits per mol of enzyme
Inhibitors: Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
Optimum pH: 10.5 – 10.8 (L-Leu→α-KⅠC), 9.4 (α-KⅠC→L-Leu)
Optimum temperature: above 70℃
pH Stability: pH 5.5−10.5 (25℃, 20hr)
Thermal stability: Below 60 ℃ (pH 6.9, 10min)