Description
SPECIFICATIONS
Product Name: L-malate: NAD+ oxidoreductase
EC: 1.1.1.37
Appearance: Slightly yellowish amorphous powder, lyophilized
Activity: Grade II, 40 U/mg-solid or more
Contaminants:
- Glutamate oxaloacetate transaminase ≤ 1.0×10-3%
- Lactate dehydrogenase ≤ 1.0×10-3%
- NADH oxidase ≤ 1.0×10-3%
Stability: Stable at – 20℃ for at least 12 months
Molecular weight: Approx. 140,000
Isoelectric point: pH 4.8±0.1
Michaelis constants:
- 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate)
- 8.1×10-6M (NADH)
Structure: 4 subunits per mole of enzyme
Inhibitors: Hg2+
Optimum pH: 8.0
Optimum temperature: 70℃
pH Stability: pH 3.0－9.0 (25℃, 20hr)
Thermal stability: Below 70℃ (pH 7.5, 15min)