Description
SPECIFICATIONS
EC 1.1.1.37
Product name: (s)-malate: NAD+ oxydoreductase
Appearance: White amorphous powder lyophilized
Activity: > 60 U/mg powder
Contaminants: Glutamate oxaloacetate transaminase ≤ 1.0×10-3 %
Lactate dehydrogenase ≤ 1.0×10-3 %
NADH oxidase ≤ 1.0×10-4 %
Stability: Stable at – 20℃ for at least 12 months
Molecular weight: Approx. 140,000
Isoelectric point: pH 4.8±0.1
Michaelis constants: 5.4×10-5M (L-Malate), 5.0×10-6 M (Oxaloacetate), 8.1×10-6M (NADH)
Inhibitors: Hg2+
Optimum pH: 8.0
Optimum temperature: 70 ℃
pH Stability: pH 3.0 – 9.0 (25 ℃, 20hr)
Thermal stability: Below 70 ℃ (pH 7.5, 15min)