Description
SPECIFICATIONS:
EC 3.4.21.26
Product name: Prolyl endopeptidase
Appearance: white amorphous powder lyophilized
Activity: Grade I, 5.0 U/mg-solid or more
Contaminants: Trypsin-like activity ≤ 0.1%
Leucine aminopeptidase ≤ 0.1%
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: Approx. 78,000
Structure: Monomer
Isoelectric point 9.1
Michaelis constants: 2.5×10-5 M (Z-Gly-Pro-MCA),
1.4×10-4 M (Z-Gly-Pro-2NNap)
Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH2Cl
Optimum pH: 6.5
Optimum temperature: 37℃ (40℃)
pH Stability: 5.5 – 8.5 (30℃, 15hr)
Thermal stability: Below 40 ℃ (pH 7.0, 10min)
Substrate specificity: Y-Pro (Ala)-X (Y, peptide or N-protected amino acid; X,
amino acid, peptide, amide, or ester)