Description
SPECIFICATIONS:
EC 3.5.1.5
Product name: Urea amidohydrolase
Appearance: white amorphous powder lyophilized
Activity: Grade II, 100U/mg-solid or more
Contaminants:
- Asparaginase < 2.0×10-2 %,
- Arginase < 2.0×10-3 %,
- NH4+ < 5.0×10-4 μg/U
Stabilizers: EDTA, glutathione, succinate, BSA
Stability: Stable at – 20 °C for at least 12 months
Molecular weight: Approx. 480,000
Isoelectric point 5.0 – 5.1
Michaelis constants: 1.05×10-2 M (Urea)
Structure: 8 active sites with SH-groups per mole of the enzyme
Inhibitors: Heavy metal ions (Ag+, Hg2+, etc.)
Optimum pH: 6.0
Optimum temperature: 60 °C
pH Stability: pH 5.5 – 8.5 (30 °C, 17hr)
Thermal stability: below 50 °C, (pH 8.0, 60min)