Description
SPECIFICATIONS
EC 3.2.1.21
Product name: b-D-glucoside glucohydrolase
Appearance: Light yellow amorphous powder lyophilized
Activity: Grade II, 10 U/mg-solid or more (containing approx. 50% of BSA)
Contaminant: α-Amylase ≤ 5.0×10-4 %
Stabilizers: Bovine serum albumin (BSA), glutathione (reduced)
Stability: Stable at －20℃ for at least 12 months
Molecular weight: approx. 110,000
Isoelectric point : 7.3
Michaelis constants: 2.8×10-3 M (p-Nitrophenyl-β-D-glucopyranoside), 3.3×10-3 M (2,4-Dichlorophenyl-β-D-glucopyranoside)
Structure 2 subunits per mol of enzyme
Optimum pH: 5.5
Optimum temperature: 50－55℃
pH Stability: pH 6.0－9.0 (25℃, 64hr)
Thermal stability: below 50℃ (pH 7.3, 1hr